Predicting disulfide connectivity from protein sequence

Advanced Computational Modelling Centre, The University of Queensland

 

If you find this service useful, please cite:
Jiangning Song, Zheng Yuan, Hao Tan, Thomas Huber and Kevin Burrage
Predicting disulfide connectivity from protein sequence using multiple
sequence feature vectors and secondary structure
Bioinformatics. 2007, 23(23):3147-3154.

 

The input to the service includes three fields.

1. Email address:
Input the email address to which the result should be sent. Please type carefully and check the email address you entered, since results will not reach you if you mis-typed your address.

 

2. The SVR models of choice:
We used two datasets to build the SVR models: SP39 dataset and SP39
template dataset. Please note that using different SVR models can lead to different prediction results of disulfide connectivity patterns. These two SVR models are provided for readers' reference and cross-validation. The sequence encoding scheme utilized here is based on PSI-BLAST profiles in the form of PSSMs, predicted secondary structure information by PSIPRED, twenty amino acid composition, the logarithmic DOC value, the cysteine ordering, the normalized protein molecular weight and the normalized sequence length.

 

3. Protein sequence:
Simply input the amino acid sequence of the query protein in one-letter format like the sample sequence given below. Protein sequences with more than 12 cysteine residues will not be processed!

 


      Email address:

     

Please select the following SVR models to predict disulfide connectivity pattern:

                   SVR-SP39 (SVR model built on SP39 dataset)
  SVR-SP39-template (SVR model built on SP39-template dataset)                

 

 

  Protein sequence [Show sample sequence]

     

     



Note that our web server will not only provide the disulfide connectivity pattern that is predicted with the highest probability score but also will return the 10 highest scoring patterns.

If you have any problems with this web server, please feel free to contact us.